Training

absorb UV light at specific

Advanced Research Laboratory

Ultraviolet (UV) spectroscopy is a technique that measures the absorption of UV                                                               . This absorption can lead to electronic transitions in molecules, making UV spectroscopy useful for identifying and analyzing substances.

Principle of UV Spectroscopy
Molecules absorb UV light at specific wavelengths, causing transitions between energy levels. The absorbance of UV light is governed by the Beer-Lambert Law, which relates absorbance to concentration and path length.

Applications of UV Spectroscopy

  • Identifying organic compounds, especially those with conjugated double bonds.
  • Quality control in the pharmaceutical industry to ensure drug purity.
  • Monitoring environmental pollutants in water and air.

Instrumentation and Measurements
A UV-Vis spectrophotometer is commonly used to measure the absorption of UV light by a sample. The resulting spectrum shows the intensity of absorption as a function of wavelength, providing valuable information about the compound.

Example Compounds and Absorption Wavelengths:

  • Benzene
    Absorption Wavelength: 254 nm
    Benzene, a simple aromatic compound, absorbs UV light due to its conjugated pi-electron system.

  • Naphthalene
    Absorption Wavelength: 220 nm
    Naphthalene shows strong UV absorption due to its extended conjugated system of double bonds.