A new RP-HPLC method was developed for the estimation 
of Rizatriptan in tablets and it was validated as per ICH guide-
lines. The chromatogram for was found to be satisfactory on sym-
metry C-18 (4.6×150mm, 5µ Thermosil column) using mobile 
phase composed of 60:40%v/v phosphate buffer of pH 3.6 and iso-
propyl alcohol at a flow rate of 1.0 ml/min. The retention time of 
Rizatriptan was found to be 7.924 min. The system suitability pa-
rameters proved that the proposed method is suitable for estima-
tion of Rizatriptan. Tailing factor for the peak was found to be 1.22 
and the theoretical plates for separation were found to be 3563. 
The method was found to be linear in the range of 10-50µg/ml. 
The precision of the method was good and the recovery of drugs is 
well within the acceptance limits of 80-120%. The LOD and LOQ 
were found to be 0.024µg/ml and 0.08 µg/ml respectively. The 
proposed RP HPLC method was found suitable for the estimation 
of Rizatriptan in formulations and is simple, selective, reproduci-
ble and accurate with good precision and can be successfully ap-
plied to routine analytical purpose.

HPLC 
Rizatriptan 
Validation 
ICH 
Dosage form

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Baghel P, Kushwaha D; Stability indicating 
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SJ; Force Degradation Study of Rizatriptan 
Benzoate by Rp-Hplc Method and Charac-
terization of Degraded Product. Biomed J 
Sci Tech Res, 1(5), 2017. DOI : 10.26717/
BJSTR.2017.01.000461  
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Analytical Method Development and Valida-

6. Kumari AS, Subhasish S, Kaushik DK, An-
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K; Development and validation of reversed-
phase high-performance liquid chromatog-
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Clin Pharmacy, 6(1), 2015. DOI 
10.4103/0976-0105.145766 
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cal formulation. Internat J Universal Phar-
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od for estimation of Etoricoxib & Paraceta-
mol and its applications in analysis of for-
mulations. Journal of Pharmacology and 
Biomedicine, 2017; 1(1): 9-14 
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(ICH). Harmonized tripartite guideline on, 
Topic Q2(R1), Validation of analytical proce-
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12. Biswas P, Jain P, Parkhe G, Mishra B. Vali-
dated HPLC method for the estimation of 
Aspirin and Omeprazole in dosage form. 
Journal of Pharmacology and Biomedicine. 
2018; 2(3): 171-180