The present work focused on preparing ethanolic extract of Melaleuca bracteata F. Muell. and evaluating its anti-inflammatory potential using in vitro and in vivo models. The ethanolic extract was dark brown in color and had an yield of 46.3% with respect to the weight of the leaf powder used for extraction. The total phenolic content in the ethanolic extract, expressed as gallic acid equivalents was found to be 74.27±0.59 GAE mg/g. The ethanolic extract of Melaleuca Bracteata exhibited the inhibition of albumin denaturation at all doses in a dose dependent manner. The 500 µg/mL concentration of the extract had shown the greatest inhibition capacity (58.9±2.869%) whereas the lowest inhibition capacity was exhibited by concentration 100 µg/mL (11.52±3.291%). MBE at dose level of 200 mg/Kg exhibited maximum reduction in edema after 3h with 27.74% reduction whereas the change decreased at the 4^th hour to 16.57%. MBE dose of 400 mg/Kg exhibited 42.77% reduction after 3h. The results obtained led to the conclusion that Melaleuca bracteata leaves are a rich source of potential phytochemicals and exhibits anti-inflammatory action.

Antiinflammatory, Melaleuca bracteata, albumin denaturation, carageenan, edema, extract

1. Ghasemian M, Owlia S, Owlia MB (2016) Review of anti-inflammatory herbal medicines. Advances Pharmacol Sci. http://dx.doi.org/10.1155/2016/9130979
2. Uttara B, Singh AV, Zamboni P, Mahajan RT (2009) Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7: 65-74.

3. Siddique S, Parveen Z, Firdaus-e-Bareen, Sania Mazhar (2020). Chemical composition, antibacterial and antioxidant activities of essential oils from leaves of three Melaleuca species of Pakistani flora. Arabian Journal of Chemistry 13: 67-74
4. Sharifi-Rad J, Salehi B, Varoni EM, Sharopov F, Yousaf Z, Ayatollahi SA, Kobarfard F, Sharifi-Rad M, Afdjei MH, Sharifi-Rad M, Iriti M (2017). Plants of the Melaleuca genus as antimicrobial Agents: From farm to pharmacy. Phytotherapy Research DOI: 10.1002/ptr.5880
5. Goswami P, Verma SK, Chauhan A, Venkatesha KT, Verma RS, Singh VR, Darokar MP, Chanotiya CS, Padalia RC (2017). Chemical composition and antibacterial activity of Melaleuca bracteata essential oil from India: A natural source of methyl eugenol. Natural Product Communication 12(6): 965-968
6. Cock IE, Winnett V, Sirdaarta J, Matthews B (2015). The potential of selected Australian medicinal plants with anti‑Proteus activity for the treatment and prevention of rheumatoid arthritis. Pharmacognosy Magazine 11(42, S1): S190-S208
7. Adesanwo KJ, Shode FO, Aiyelaagbe OO, Rabiu OO, Oyede RT, Oluwole FS (2009). Antisecretory and antiulcerogenic activities of the stem bark extract of Melaleuca bracteata and isolation of principles. Journal of Medicinal Plants Research 3(10): 822-824

1. Singh AP, Mishra B (2020). Evaluation of anti-inflammatory potential of Rutin using in vitro models. Journal of Pharmacology and Biomedicine 4(1): 211-217
2. Amdekar S, Roy P, Singh V, Kumar A, Singh R, Sharma P (2012). Anti-Inflammatory Activity of Lactobacillus on Carrageenan-Induced Paw Edema in Male Wistar Rats. International Journal of Inflammation doi:10.1155/2012/752015

10. Saso L, Valentini G, Casini ML, Grippa E, Gatto MT, Leone MG (2001). Inhibition of heat-induced denaturation of albumin by nonsteroidal antiinflammatory drugs (NSAIDs): Pharmacological implications. Archives of Pharmacal Research 24(2): 150-158.
11. Opie EL (1962). On the relation of necrosis and inflammation to denaturation of proteins. Journal of Experimental Medicine 115: 597-608