Rutin is a secondary plant metabolite known to possess excellent antioxidant potential. The present work was undertaken with an objective to evaluate the anti-inflammatory potential of rutin using in vitro models. Inhibition of albumin denaturation and antiprotease action were used as the models for studying the anti-inflammatory action. The capacity of different concentrations of rutin to inhibit protein denaturation of albumin was ranging from 27.33 ± 0.001% to 67.82 ± 0.002 comparable to ibuprofen (standard). On the other hand the capacity of different concentrations of rutin to inhibit serine protease was found to be ranging from 20.36 ± 0.214% to 77.53 ± 0.138 in the assay and therefore reinforces for its tremendous anti inflammatory property.

Rutin, albumin denaturation, antiprotease, anti-inflammatory, in vitro, chemical mediators

Ghasemian M, Owlia S, Owlia MB. Review of anti-inflammatory herbal medicines. Advances Pharmacol Sci. 2016; http://dx.doi.org/10.1155/2016/9130979 2. Uttara, B., Singh, A.V., Zamboni, P. and Mahajan, R.T. Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol. 2009; 7: 65-74. 3. Marienfeld R, Neumann M, Chuvpilo S, Escher C, Kneitz B, Avots A, et al. Cyclosporin A interferes with the inducible degradation of NF-k B inhibitors, but not with the processing of p105/NF-k B1 in T cells. Eur J Immunol. 1997; 27:1601–9. 4. Borrelli F, Romano B, Petrosino S, Pagano E, Capasso R, Coppola D, Battista G, Orlando P, Di Marzo V, Izzo AA (2015). Palmitoylethanolamide naturally occurring lipid an orally effective intestinal anti- inflammatory agent. British J Pharmacol, 172: 142-158 5. Leelaprakash G, Mohan Dass S (2011). In vitro anti inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development & Research, 3: 189-196 6. Kohli K, Ali J, Ansari MJ, Raheman Z (2005). Curcumin: A natural anti inflammatory agent. Indian J Pharmacol, 37: 141-147 7. Achoui M, Appleton D, Abdulla MA, Awang K, Mohd MA, et al. (2010) In Vitro and In Vivo Anti-Inflammatory Activity of 17-O- Acetylacuminolide through the Inhibition of Cytokines, NF-kB Translocation and IKKb Activity. PLoS ONE 5(12): e15105. doi:10.1371/journal.pone.0015105 8. Halliwell, B. and Gutteridge, J.M. (2015) Free Radicals in Biology and Medicine. Oxford University Press, USA. 9. Jayameena P, Sivakumari K, Ashok K, Rajesh S (2018). In vitro anti-inflammatory (membrane stabilization) and antioxidant potential of rutin. Res J Life Sci Bioinformatics Pharm Chem Sci, 4: 265-274 10. Kim H, Kong H, Choi B, Yang Y, Kim Y, Lim MJ, Neckers L, Jung Y (2005). Metabolic and pharmacological properties of rutin, a dietary quercetin glycoside, for treatment of inflammatory bowel disease. Pharm Res, 22: 1499-1509 11. Osman NI, Sidik NJ, Awal A, Adam NAM, Rezali NI (2016). In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-infl ammatory use in gouty arthritis. J Intercult Ethnopharmacol, 5: 343-349. 12. Kumari S, Yasmin N, Hussain MR, Babuselvam M (2015). In vitro antiinflammatory and anti-arthritic property of Rhizopora mucronata leaves. Internat J Pharm Sci Res, 6: 482-485. 13. Oyedepo OO and Femurewa AJ (1995). Anti‐protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Internat J Pharmacog, 33: 65‐69. 14. Sakat S, Juvekar AR, Gambhire MN (2010). In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Internat J Pharma Pharmacological Sc, 2: 146-155. 15. Saso L, Valentini G, Casini ML, Grippa E, Gatto MT, Leone MG (2001). Inhibition of heat-induced denaturation of albumin by nonsteroidal antiinflammatory drugs (NSAIDs): Pharmacological implications. Arch Pharm Res, 24: 150-158. 16. Opie EL (1962). On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med, 115: 597-608. 17. Das S, Chatterjee S (1995). Long term toxicity study of ART-400. Indian Indg Med, 16: 117- 123. 18. Dai H, Korthuis RJ (2011). Mast cell proteases and inflammation. Drug Discov Today Dis Models, 8: 47-55. doi: 10.1016/j.ddmod.2011.06.004