Successive solvent extraction of the defatted bark of Annona squamosa was perfomed using soxhlet method and the extraction abilities of different solvents for recovering extractable components from leaves followed the order: ethanol>ethylacetate>water. The findings of preliminary phytochemical screening suggest the presence of alkaloids, saponins, phenolics, tannins, terpenoids, sterols, and flavonoids in the bark of the plant. The acute toxicity study reveals a LD₅₀ of more than 2000 mg/Kg for the phytochemical rich ethanolic extract of the bark of AS. The ethanolic extract of Annona squamosa bark was evaluated for the in vivo wound healing effect by the excision model. The topical application of 1 and 2 % w/w of the Annona squamosa extract resulted in an accelerated wound healing capability.  The plant extract (ASBE 2%) exhibited 86.15 % contraction of wound on the 15^th day which was more than the povidone iodine used as the standard that exhibited 78.1% contraction of the wound. The control animals with ointment base were found to contraction 69.42 % while ASBE 1% healed 78.1% of wound by the 15^th day.

Annona squamosa, wound, bark extract, phytochemical, phenolics, extraction

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