The objective of the present study was to prepare phytosomal formulation loaded with hydroalcoholic extract of Annona squamosa leaf. The extraction of leaf powder was done by maceration technique using 10:90, water: ethanol mixture with an extraction yield of 11.3%.  The extract was found to contain alkaloids, glycosides, proteins, phenols, flavonoids and triterpenes. Phytosomes of the extract were prepared by solvent evaporation method using lecithin as the lipid molecule. The particle size of the phytosomes was from 678 nm to 2617 nm in size with a polydispersity index varying between 0.368 - 0.718. The phytosomes were visible as rigid, spherical vesicles in SEM image. The surface of the phytosome vesicles was found to be regular and smooth. Sharp and distinct endothermic peaks in DSC revealed the formulation of stable phytosomes due to molecular interactions between the extract and lecithin. The phytosomes exhibited anti-inflammatory action in the in vitro assays by inhibiting albumin denaturation and protease activity. The phytosomes were found to be stable under the storage conditions. The best formulation with respect to particle size and anti-inflammatory action was ASP 4 that contained 2:0.5 ratio of lecithin: extract.

Phytosome, Annona squamosa, extract, lecithin, anti-inflammatory

1. Ahmad S. 2007. Pharmacognosy-Introduction of Plant Constituents and their Tests. 1-40. (Available at http://nsdl.niscair.res.in/handle/123456789/708 Accessed on 18 May 2021).
2. Bombardelli E. P. Complexes Between Phospholipids and Vegetal Derivatives of Biological Interest. Fitoterapia. 1989; 60:1-9.
3. Dhase AS, Saboo SS. Preparation and Evaluation of Phytosomes Containing Methanolic Extract of Leaves of Aegle Marmelos (Bael). International Journal of PharmTech Research 2015; 8(6): 231-240
4. Govt. of India, Ministry of Health & Family Welfare. Indian Pharmacopoeia. Ghaziabad: The Indian Pharmacopoeial Commission, 2007.
5. Karole S, Gautam GK, Gutpa S. Preparation and evaluation of phytosomes containing ethanolic extract of leaves of Bombax ceiba for hepatoprotective activity. The Pharma Innovation Journal 2019; 8(2): 22-26
6. Khandelwal, K.R., 2005. Practical Pharmacognosy-Techniques and Experiments, 14th ed. NiraliPrakashan, Pune, pp. 153
7. Kidd P, Head K. A review of the bioavailability and clinical efficacy of milk thistle phytosomes: a Silybin phosphatidylcholine complex. Alternative Medicine Reviews. 2005; 10(3): 193-203.
8. Kidd PM, Phytosomes, Highly Bioavailable Plant Extracts.[Online].[Cited 2008 Dec 12]. Available from://www.dockidd.com /pdf/ 2Links PhytosomeInd ,5_10_04.pdfstifolia
9. Kumari S, Yasmin N, Hussain MR, Babuselvam M (2015). In vitro antiinflammatory and anti-arthritic property of Rhizopora mucronata leaves. International J Pharm Sci Res 2015, 6, 482-485.
10. Nadkarni KM, Nadkarni AK. Indian Materia Medica. Bombay: Popular Prakashan Private Ltd; 1976.
11. Oyedepo OO and Femurewa AJ. Anti‐protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. International J Pharmacogn 1995, 33, 65‐69.
12. Patwardhan B., Vaidya A.D.B and Chorghade M (2004) “Ayurveda and Natural Products Drug Discovery” Current Science 86(6):789-799.
13. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International J Pharma Pharmacological Sci 2010, 2, 146-155.
14. Singh AP, Mishra B. Evaluation of anti-inflammatory potential of Rutin using in vitro models. J Pharmacol Biomed, 2020, 4(1), 211-217