Nanoparticles loaded with Repaglinide were prepared using gelatin as the polymeric matrix and glutaraldehyde, PVP, PEG 2000 and PEG 10000 as the cross linking agents. A method of desolvation precipation was used for obtaining the nanoparticles, using acetone as the desolvating agent in each formulation. All the formulations (F1 to F4) were characterized for yield, entrapment efficiency, particle size and in vitro drug release. The order of the particle size obtained using various cross-linking agents was glutaraldehyde < PVP < PEG2000 < PEG 10000. The highest particles size was obtained in F4 (2228.37 nm) while the lowest was in F1 (242.73 nm). The yield of the nanoparticles was also affected by the choice of the cross-linking agent and ranged from 56-74%. The drug entrapment efficiencies of different formulations were in the range of 63.24 to 38.52 %. Glutaraldehyde and PVP were able to sustain the relesae of repaglinide for upto 24 h and 12 h respectively whereas PEG 2000 and PEG 10000 formualations released drug within 8 and 6 h of the study respectively.

Repaglinide , Nanoparticles , Desolvation , Gelatin , Release , Entrapment

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