Trigonella foenum-graecum, Zingiber officinale and Piper nigrum were extracted using ethanol and incorporated in to liposomes. These liposomes were formulated as gel formulation for topical application for management of gout pain. The extraction yield for TF, ZO and PN were found to be 28.7, 37.4 and 21.1% respectively respectively. The total phenolic content in TF, ZO and PN extracts were found to be 28.3 ± 1.301, 13.2 ± 1.196 and 9.8 ± 0.894 GAE mg/100g whereas it was found to be 38.6 ± 0.735, 51.4 ± 0.721 and 63.08 ± 0.823 GAE mg/100g for 1:1:1, 1:2:1 and 2:1:1 mixture ratio of TF, ZO and PN respectively. The particle size of the liposomes was found to decrease with an increase in sonication time but the sonication time did not affect the total phenolic content of the liposomes. The particle size of the liposome formulated using 5 min sonication time was considered optimum and was found to be 4.27 ± 0.033µm. The liposomes were stable at both the temperature conditions and the particle size of the liposomes did not change considerably over a period of three months. ELF3 loaded gel formulations were prepared with different ratio of ELF3 using carbopol 934. The pH of all the formulations was in the range of the pH of the skin (5.0-5.5) and they exhibited optimum viscosity. The pH, viscosity and spreadability of PHLG3 was 5.3, 1694 cp & 62 mm respectively.

Liposome, polyherbal, carbopol, gout, pain, extract, phenolic

1. Appendix 13.1 Buffers, In: Indian Pharmacopoeia, 1996, The Indian Pharmacopoeia Commission, Ghaziabad
2. Armann C, Roding J, Ghyczy M, Pratzel HG. Liposomes from soya phospholipids as percutaneous drug carriers. Arzneim-Forsch/ Drug Research 1990; 40: 1365-1368
3. Bang JS, Oh DH, Choi HM, Sur B-J, Lim S-J, Kim JY, Yang H-I Yoo MC, Hahm D-H, Kim KS. Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1β-stimulated fibroblast-like synoviocytes and in rat arthritis models. Arthritis Research and Therapy 2009; 11: R46. doi:10.1186/ar2662
4. Chanthasri W, Puangkeaw N, Kunworarath N, Jaisamut P, Limsuwan S, Maneenoon K, Choochana P, Chusri S. Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand. BMC Complementary and Alternative Medicine 2018; 18:73. https://doi.org/10.1186/s12906-018-2131-y
5. Dag D, Oztop MH. Formation and Characterization of Green Tea Extract Loaded Liposomes. Journal of Food Science 2017; doi: 10.1111/1750-3841.13615
6. Ehrlich SD. Herbal medicine. 2011. [Last accessed on 2019 November 1]. Available from: http://www.umm.edu/health/medical/altmed/treatment/herbal-medicine .
7. Goyal S, Gupta N, Chatterjee S. Investigating therapeutic potential of Trigonella foenum-graecum L. as our defense mechanism against several human diseases. Journal of Toxicology 2016; http://dx.doi.org/10.1155/2016/1250387
8. https://en.wikipedia.org/wiki/Ginger;  assessed on 2 November 2019
9. Jain S, Kale DP, Swami R, Katiyar SS. Codelivery of benzoyl peroxide & adapalene using modified liposomal gel for improved acne therapy. Nanomedicine 2018; 13(12): 1481-1493
10. Kamboj VP. Herbal medicine. Current Science 2000; 78: 35–51.