Abstract Article


Screening of antidepressant potential of leaves of Araucaria columaris

Yogesh Mathur, Amit Jain

ONLINE ISSN : 2456-8244



The present work was undertaken with an objective to establish the anti-depressant action of ethanolic leaf extract of Araucaria columnaris. The yield of the ethanolic extract was found to be 36.9% and the extract was black in color. The preliminary phytochemical analysis suggests the presence of cardiac glycosides, phenolics, terpenoids, sterols, and flavonoids in the leaves. The antidepressant action of the extract (EEAC) was tested using two animal models at two dose levels (200 and 400 mg/kg) using forced swim test (FST) and tail suspension test (TST) models. The immobility time in the TST for EEAC (400 mg/kg) was found to be 18.33 ± 1.994 sec and was significantly comparable (p<0.0001) to that of fluoxetine at a dose on 10mg/kg (9.67 ± 0.667 sec). The swimming frequency in the FST increased significantly at both the dose levels of EEAC and it surpassed fluoxetine (43.00 ± 1.211) at 400 mg/kg dose of EEAC (44.00 ± 1.317).


Araucaria columnaris, extract, forced swim test, tail suspension test, antidepressant, fluoxetine



  1. Aslam MS, Choudhary BA, Ijaz AS, Uzair M, Ahmad MS, Mamat AS (2015) Biological screening of Araucaria columnaris. International Journal of Pharmacy and Pharmaceutical Sciences. 7(4): 313-315
  2. Aslam MS, Choudhary BA, Uzair M, Ijaz AS (2013) Phytochemical and ethno-pharmacological review of the genus Araucaria-review. Tropical Journal of Pharmaceutical Research. 12(4): 651-659
  3. Aslam MS, Choudhary BA, Uzair M, Ijaz AS (2014) Phytochemistry of aerial parts of araucaria columnaris. Journal of Applied Pharmacy. 6(1): 114-120
  4. de Oliveira KN, Costa P, Santin JR, Mazzambani L, Bürger C, Mora C, Nunes RJ, de Souza MM (2011) Synthesis and antidepressant-like activity evaluation of sulphonamides and sulphonyl-hydrazones. Bioorganic & Medicinal Chemistry. 19: 4295-4306
  5. Guan LP, Zhao D-H, Chang Y, Wen Z-S, Tang L-M, Huang F-F (2013) Synthesis of 2,4-dihydroxychalcone derivatives as potential antidepressant effect. Drug Research. 63: 46-51
  6. https://www.gardenia.net/plant/araucaria-columnaris?; assessed on 22/04/2021
  7. Malviya M, Mishra B, Korde B (2021) Antidepressant molecules: Synthesis and evaluation of novel azetidinone compounds. Journal of Pharmacology and Biomedicine. 5(2): 286-294.
  8. Saranya Devi K, Rathinamala J, Jayashree S (2014) Phytochemical and therapeutic effect of araucaria columnaris resin extracts on clinical pathogens. European Journal of Molecular Biology and Biochemistry. 1(5): 182-185
  9. Scapagnini G, Davinelli S, Drago F, De Lorenzo A, Oriani G (2012) Antioxidants as antidepressants: fact or fiction? CNS Drugs. 26(6): 477-490. doi: 10.2165/11633190-000000000-00000. PMID: 22668245.
  10. Schmeda-Hirschmann G, Astudillo L, Sepulveda B, Rodrıguez JA, Theoduloz C, Yanez T, Palenzuela JA (2005) Gastroprotective effect and cytotoxicity of natural and semisynthetic labdane diterpenes from araucaria araucana resin. Zeitschrift für Naturforschung C. 60(7-8): 511-522
  11. Shabir G, Anwar F, Sultana B, Khalid ZM, Afzal M, Khan QM, Ashrafuzzaman M (2011) Antioxidant and Antimicrobial Attributes and Phenolics of Different Solvent Extracts from Leaves, Flowers and Bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf.]. Molecules. 7302-7319. doi:10.3390/molecules16097302
  12. Tiwari P, Joshi A, Dubey BK (2017) Total phenolic content, flavonoid concentration, antimicrobial and insecticidal screening of aqueous extracts of Annona squamosa (seeds), Azadirachta indica (leaves) and Lavandula angustifolia (flower). Journal of pharmacology and Biomedicine. 1(1): 30-43
  13. Zaffar M, Munis MFH, Rasul F, Masood S, Hassan SW, Imran M, Nasim W, Zakir A, Quraishi UM, Chaudhary HJ (2014) Biological assays of plant extracts from Araucaria columnaris and Cycas revoluta. Journal of Food, Agriculture & Environment.  12(1): 128-131