Validated HPLC method for the estimation of Aspirin and Omeprazole in dosage form
Pranita Biswas, Prabhat Jain, Geeta Parkhe, Brijeshkunvar Mishra*
ONLINE ISSN : 2456-8244ABSTRACT
A new simple, rapid, selective, precise and accurate high-performance liquid chromatographic method
has been developed for the estimation of aspirin and omeprazole in marketed formulation. The RPHPLC
method was developed for estimation of aspirin and omeprazole in bulk and tablet dosage
form by isocratically using 20 mM KH2PO4: acetonitrile (pH 3.5) in the ratio of 20:80 v/v as mobile
phase, Thermo C-18 column (4.6 x 250 mm, 5μ particle size) column as stationary phase and
chromatogram was recorded at 275 nm. Then developed method was validated by using various
parameters. The parameters linearity, precision, accuracy, robustness, limit of detection and limit of
quantitation were studied according to International Conference on Harmonization guidelines. The
proposed method was found to be accurate, repeatability and consistent. It was successfully applied
for the analysis of the drug in marketed formulation and could be effectively used for the routine
analysis of formulation containing the drug without any alteration in the chromatography conditions.
KEYWORDS
Aspirin, Omeprazole, HPLC, Chromatography, Spectrophotometry
DOI
REFERENCES
1. Swethanagini V, Vasanth Kumar. M.
Validated RP HPLC Method for
Simultaneous Estimation of
Omeprazole and Cinitapride in
Combined Dosage Forms. I J R P C.
2012; 2(4): 1078-1085.
2. Chodvadiya J, Chaitanyakumar K,
Maheshwari D.et al Simultaneous
Estimation Of Aspirin And
Lansoprazole By RP –HPLC Method Int J R Sci Res. 2015; 6(4): 3385 -
3390.
3. Kayesh PR, Rahman A, Sultan M, et
al. Development and Validation of a
RP -HPLC Method for the
Quantification of Omeprazole in Pharmaceutical Dosage Form. J. Sci.
Res. 2013; 5(2): 335 -3425.
4. International Conference on
Harmonization (ICH). Harmonized
tripartite guideline on, Topic Q2(R1),
Validation of analytical procedures:
Text and methodology 2005.